Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.

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Previous studies have demonstrated that the ability of A. The process of programmed cell death is called apoptosis. The heritability of proline was the highest 0.

Gour Central University, Sagar M. Andrographis paniculata is an annual herb belongs to family Acanthaceae. Anthelmintic potential of Andrographis paniculataCajanus cajan and Silybum marianum. Phyto chemical analysis of hydroalcoholic extract of A. Introduction Salinity is a major abiotic stress that causes important alterations in the plant growth and development.

Solvents AR and chromatography grade for isolation and purification of the compounds were used as supplied by Fisher Scientific Leicestershire, Ancrographis. The combination of both the plants 1: Reportedly, improving the content of some phytochemicals such as flavonoids as one of the most important pharmaceutical contents could be enhanced through salinity and drought stresses [ 121415 ].

Andrographis paniculata extract was found most effective wctivity Ovcar cell line Many of those metabolic changes such as sucrose, oligosaccharides, polyhydric alcohols, proline, and polyamines are observable in the leaves of plants exposed to abiotic stresses [ 4 ]. The following drugs and chemicals were used: The correlative aspects of the three anticancer phytochemicals production as well as the accumulation of proline in the tolerant and sensitive A.

Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress

Culturing of cell line: The stock solution was diluted with the same solvent to produce different concentration of working standards 0.


The filtrate was evaporated to dryness using a rotary evaporator yield was This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

After 30 days of culturing almost in 70 days oldthe plants were placed in different salinity levels. Analysis of variance showed high significant differences among the accessions for AG and NAG contents. All chemicals used were of analytical grade. View at Google Scholar D. We noticed that the major difference among the tolerant and sensitive accessions was related to their ability in tolerating high salt stress. Diterpene lactones are phytochemical structures, which could be found in different parts of this medicinal herb.

Apart from this, alkaloids were present only in the extract of Andrographis paniculata while, saponins were present only in Silybum marianum extract Table 1. The slight differences in retention times RT anticanceg the phytochemicals are due to the running of the samples in different batches using a refreshed mobile phase.

The dry weight was used to monitor the physiological response of each accession to salinity. Recent studies have reported antitumor effects of the flavonoids, quercetin, genistein and baicalein obtained from plant extracts Shoeb et al.

Interestingly, the correlation between proline as well as NAG with the other measured morphological characteristics was andrograpihs and positive in the salt-stressed plants. After 24 h, the cells were treated with the extracts.

Among cancer especially prostrate cancer has been responsible for drastic mortality rate in males.

The trend of proline and the three main phytochemicals in tolerant accession were linear and positive, while in andrographid accession they were nonlinear. This finding matched up with the Chutipaijit et al. These compounds are produced mainly from aerial organs of the plant, especially leaves. The comparison of decrease in studied morphological traits in tolerant and sensitive accessions of A.


BioMed Research International

How to cite this article: Effects of salinity levels on TDW, proline and three main phytochemicals in tolerant and sensitive accession of A. This can be utilized as a potential in the next breeding programs to develop the salt-tolerant varieties in A. Bounded stain was solubilised with Tris buffer 10 mM and the optical densities were read on an automated spectrophotometric plate reader Molecular Devices, USA at a wavelength of nm, Optical density OD of SRB in each well is directly proportional to the cell number.

The colorimetric assay estimated cell number indirectly by staining total cellular protein with the dye SRB. A pilot double blind study. In conclusions, the result supports the use of the plant as described in folk medicine, that the aerial plant parts can be anddrographis to treat cancer.

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Single-cell suspensions were prepared by the treatment of cells with 0. In our pniculata, we have carried out an effort towards checking the effect of Kalmegh extracts towards restoring the expression of tumor suppressor gene, E-cadherin CDH1 and p in PC-3 cell line.

Salinity causes the adverse effects in all physiological processes of plants. The effects of salinity levels on studied traits among the four accessions of A.